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( a-c ) Heatmaps displaying growth displayed as area under curve in selected carbon sources for S . Tm in comparison with K. oxytoca MK01 WT and the sKO mutant after 24 h aerobic incubation using the Biolog <t>microarrays</t> <t>PM1</t> and PM2a. Sugars are grouped based on their chemical structure with simple sugars and sugar alcohols displayed in ( a ), sugar acids, amines and ketones displayed in ( b ) and amino acids and nucleosides displayed in ( c ). Color scale bar shows AUC over the period of 24 h aerobic incubation. ( a-c ) Results represent mean (average growth) values of n = 3 independent measurements. ( d-g ) Growth curves of S . Tm and MK01 WT and sKO in selected carbon sources including ( d ) dulcitol, ( e ) raffinose, ( f ) sucrose and ( g ) tagatose. Results represent mean ± SEM growth values of n = 3 independent measurements with n = 2 technical replicates. ( h ) S . Tm CFUs and ( i ) K. oxytoca / E. coli CFUs in MM9 medium supplemented with 5 g/l or 10 g/l dulcitol after 24 h of co-cultivation in a 1:1 ratio. Results represent mean ± SEM of n = 2 independent experiments performed in n = 2 technical replicates.
Biolog Microarrays Pm1, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( a-c ) Heatmaps displaying growth displayed as area under curve in selected carbon sources for S . Tm in comparison with K. oxytoca MK01 WT and the sKO mutant after 24 h aerobic incubation using the Biolog <t>microarrays</t> <t>PM1</t> and PM2a. Sugars are grouped based on their chemical structure with simple sugars and sugar alcohols displayed in ( a ), sugar acids, amines and ketones displayed in ( b ) and amino acids and nucleosides displayed in ( c ). Color scale bar shows AUC over the period of 24 h aerobic incubation. ( a-c ) Results represent mean (average growth) values of n = 3 independent measurements. ( d-g ) Growth curves of S . Tm and MK01 WT and sKO in selected carbon sources including ( d ) dulcitol, ( e ) raffinose, ( f ) sucrose and ( g ) tagatose. Results represent mean ± SEM growth values of n = 3 independent measurements with n = 2 technical replicates. ( h ) S . Tm CFUs and ( i ) K. oxytoca / E. coli CFUs in MM9 medium supplemented with 5 g/l or 10 g/l dulcitol after 24 h of co-cultivation in a 1:1 ratio. Results represent mean ± SEM of n = 2 independent experiments performed in n = 2 technical replicates.
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( a-c ) Heatmaps displaying growth displayed as area under curve in selected carbon sources for S . Tm in comparison with K. oxytoca MK01 WT and the sKO mutant after 24 h aerobic incubation using the Biolog <t>microarrays</t> <t>PM1</t> and PM2a. Sugars are grouped based on their chemical structure with simple sugars and sugar alcohols displayed in ( a ), sugar acids, amines and ketones displayed in ( b ) and amino acids and nucleosides displayed in ( c ). Color scale bar shows AUC over the period of 24 h aerobic incubation. ( a-c ) Results represent mean (average growth) values of n = 3 independent measurements. ( d-g ) Growth curves of S . Tm and MK01 WT and sKO in selected carbon sources including ( d ) dulcitol, ( e ) raffinose, ( f ) sucrose and ( g ) tagatose. Results represent mean ± SEM growth values of n = 3 independent measurements with n = 2 technical replicates. ( h ) S . Tm CFUs and ( i ) K. oxytoca / E. coli CFUs in MM9 medium supplemented with 5 g/l or 10 g/l dulcitol after 24 h of co-cultivation in a 1:1 ratio. Results represent mean ± SEM of n = 2 independent experiments performed in n = 2 technical replicates.
Phenotype Microarray 96 Well Plates Pm1, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( a-c ) Heatmaps displaying growth displayed as area under curve in selected carbon sources for S . Tm in comparison with K. oxytoca MK01 WT and the sKO mutant after 24 h aerobic incubation using the Biolog <t>microarrays</t> <t>PM1</t> and PM2a. Sugars are grouped based on their chemical structure with simple sugars and sugar alcohols displayed in ( a ), sugar acids, amines and ketones displayed in ( b ) and amino acids and nucleosides displayed in ( c ). Color scale bar shows AUC over the period of 24 h aerobic incubation. ( a-c ) Results represent mean (average growth) values of n = 3 independent measurements. ( d-g ) Growth curves of S . Tm and MK01 WT and sKO in selected carbon sources including ( d ) dulcitol, ( e ) raffinose, ( f ) sucrose and ( g ) tagatose. Results represent mean ± SEM growth values of n = 3 independent measurements with n = 2 technical replicates. ( h ) S . Tm CFUs and ( i ) K. oxytoca / E. coli CFUs in MM9 medium supplemented with 5 g/l or 10 g/l dulcitol after 24 h of co-cultivation in a 1:1 ratio. Results represent mean ± SEM of n = 2 independent experiments performed in n = 2 technical replicates.
Phenotype Microarray Carbon Microplate Pm1, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( a-c ) Heatmaps displaying growth displayed as area under curve in selected carbon sources for S . Tm in comparison with K. oxytoca MK01 WT and the sKO mutant after 24 h aerobic incubation using the Biolog <t>microarrays</t> <t>PM1</t> and PM2a. Sugars are grouped based on their chemical structure with simple sugars and sugar alcohols displayed in ( a ), sugar acids, amines and ketones displayed in ( b ) and amino acids and nucleosides displayed in ( c ). Color scale bar shows AUC over the period of 24 h aerobic incubation. ( a-c ) Results represent mean (average growth) values of n = 3 independent measurements. ( d-g ) Growth curves of S . Tm and MK01 WT and sKO in selected carbon sources including ( d ) dulcitol, ( e ) raffinose, ( f ) sucrose and ( g ) tagatose. Results represent mean ± SEM growth values of n = 3 independent measurements with n = 2 technical replicates. ( h ) S . Tm CFUs and ( i ) K. oxytoca / E. coli CFUs in MM9 medium supplemented with 5 g/l or 10 g/l dulcitol after 24 h of co-cultivation in a 1:1 ratio. Results represent mean ± SEM of n = 2 independent experiments performed in n = 2 technical replicates.
Phenotypic Microarrays Pm1, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( a-c ) Heatmaps displaying growth displayed as area under curve in selected carbon sources for S . Tm in comparison with K. oxytoca MK01 WT and the sKO mutant after 24 h aerobic incubation using the Biolog <t>microarrays</t> <t>PM1</t> and PM2a. Sugars are grouped based on their chemical structure with simple sugars and sugar alcohols displayed in ( a ), sugar acids, amines and ketones displayed in ( b ) and amino acids and nucleosides displayed in ( c ). Color scale bar shows AUC over the period of 24 h aerobic incubation. ( a-c ) Results represent mean (average growth) values of n = 3 independent measurements. ( d-g ) Growth curves of S . Tm and MK01 WT and sKO in selected carbon sources including ( d ) dulcitol, ( e ) raffinose, ( f ) sucrose and ( g ) tagatose. Results represent mean ± SEM growth values of n = 3 independent measurements with n = 2 technical replicates. ( h ) S . Tm CFUs and ( i ) K. oxytoca / E. coli CFUs in MM9 medium supplemented with 5 g/l or 10 g/l dulcitol after 24 h of co-cultivation in a 1:1 ratio. Results represent mean ± SEM of n = 2 independent experiments performed in n = 2 technical replicates.
Phenotypic Microarrays Pm1 10 Plates, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( a-c ) Heatmaps displaying growth displayed as area under curve in selected carbon sources for S . Tm in comparison with K. oxytoca MK01 WT and the sKO mutant after 24 h aerobic incubation using the Biolog <t>microarrays</t> <t>PM1</t> and PM2a. Sugars are grouped based on their chemical structure with simple sugars and sugar alcohols displayed in ( a ), sugar acids, amines and ketones displayed in ( b ) and amino acids and nucleosides displayed in ( c ). Color scale bar shows AUC over the period of 24 h aerobic incubation. ( a-c ) Results represent mean (average growth) values of n = 3 independent measurements. ( d-g ) Growth curves of S . Tm and MK01 WT and sKO in selected carbon sources including ( d ) dulcitol, ( e ) raffinose, ( f ) sucrose and ( g ) tagatose. Results represent mean ± SEM growth values of n = 3 independent measurements with n = 2 technical replicates. ( h ) S . Tm CFUs and ( i ) K. oxytoca / E. coli CFUs in MM9 medium supplemented with 5 g/l or 10 g/l dulcitol after 24 h of co-cultivation in a 1:1 ratio. Results represent mean ± SEM of n = 2 independent experiments performed in n = 2 technical replicates.
Biolog Phenotyping Microarray 1 Plate, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( a-c ) Heatmaps displaying growth displayed as area under curve in selected carbon sources for S . Tm in comparison with K. oxytoca MK01 WT and the sKO mutant after 24 h aerobic incubation using the Biolog <t>microarrays</t> <t>PM1</t> and PM2a. Sugars are grouped based on their chemical structure with simple sugars and sugar alcohols displayed in ( a ), sugar acids, amines and ketones displayed in ( b ) and amino acids and nucleosides displayed in ( c ). Color scale bar shows AUC over the period of 24 h aerobic incubation. ( a-c ) Results represent mean (average growth) values of n = 3 independent measurements. ( d-g ) Growth curves of S . Tm and MK01 WT and sKO in selected carbon sources including ( d ) dulcitol, ( e ) raffinose, ( f ) sucrose and ( g ) tagatose. Results represent mean ± SEM growth values of n = 3 independent measurements with n = 2 technical replicates. ( h ) S . Tm CFUs and ( i ) K. oxytoca / E. coli CFUs in MM9 medium supplemented with 5 g/l or 10 g/l dulcitol after 24 h of co-cultivation in a 1:1 ratio. Results represent mean ± SEM of n = 2 independent experiments performed in n = 2 technical replicates.
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( a-c ) Heatmaps displaying growth displayed as area under curve in selected carbon sources for S . Tm in comparison with K. oxytoca MK01 WT and the sKO mutant after 24 h aerobic incubation using the Biolog <t>microarrays</t> <t>PM1</t> and PM2a. Sugars are grouped based on their chemical structure with simple sugars and sugar alcohols displayed in ( a ), sugar acids, amines and ketones displayed in ( b ) and amino acids and nucleosides displayed in ( c ). Color scale bar shows AUC over the period of 24 h aerobic incubation. ( a-c ) Results represent mean (average growth) values of n = 3 independent measurements. ( d-g ) Growth curves of S . Tm and MK01 WT and sKO in selected carbon sources including ( d ) dulcitol, ( e ) raffinose, ( f ) sucrose and ( g ) tagatose. Results represent mean ± SEM growth values of n = 3 independent measurements with n = 2 technical replicates. ( h ) S . Tm CFUs and ( i ) K. oxytoca / E. coli CFUs in MM9 medium supplemented with 5 g/l or 10 g/l dulcitol after 24 h of co-cultivation in a 1:1 ratio. Results represent mean ± SEM of n = 2 independent experiments performed in n = 2 technical replicates.
Microarray Plates Pm1, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( a-c ) Heatmaps displaying growth displayed as area under curve in selected carbon sources for S . Tm in comparison with K. oxytoca MK01 WT and the sKO mutant after 24 h aerobic incubation using the Biolog <t>microarrays</t> <t>PM1</t> and PM2a. Sugars are grouped based on their chemical structure with simple sugars and sugar alcohols displayed in ( a ), sugar acids, amines and ketones displayed in ( b ) and amino acids and nucleosides displayed in ( c ). Color scale bar shows AUC over the period of 24 h aerobic incubation. ( a-c ) Results represent mean (average growth) values of n = 3 independent measurements. ( d-g ) Growth curves of S . Tm and MK01 WT and sKO in selected carbon sources including ( d ) dulcitol, ( e ) raffinose, ( f ) sucrose and ( g ) tagatose. Results represent mean ± SEM growth values of n = 3 independent measurements with n = 2 technical replicates. ( h ) S . Tm CFUs and ( i ) K. oxytoca / E. coli CFUs in MM9 medium supplemented with 5 g/l or 10 g/l dulcitol after 24 h of co-cultivation in a 1:1 ratio. Results represent mean ± SEM of n = 2 independent experiments performed in n = 2 technical replicates.
Biolog Microarray Plates Pm1, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( a-c ) Heatmaps displaying growth displayed as area under curve in selected carbon sources for S . Tm in comparison with K. oxytoca MK01 WT and the sKO mutant after 24 h aerobic incubation using the Biolog microarrays PM1 and PM2a. Sugars are grouped based on their chemical structure with simple sugars and sugar alcohols displayed in ( a ), sugar acids, amines and ketones displayed in ( b ) and amino acids and nucleosides displayed in ( c ). Color scale bar shows AUC over the period of 24 h aerobic incubation. ( a-c ) Results represent mean (average growth) values of n = 3 independent measurements. ( d-g ) Growth curves of S . Tm and MK01 WT and sKO in selected carbon sources including ( d ) dulcitol, ( e ) raffinose, ( f ) sucrose and ( g ) tagatose. Results represent mean ± SEM growth values of n = 3 independent measurements with n = 2 technical replicates. ( h ) S . Tm CFUs and ( i ) K. oxytoca / E. coli CFUs in MM9 medium supplemented with 5 g/l or 10 g/l dulcitol after 24 h of co-cultivation in a 1:1 ratio. Results represent mean ± SEM of n = 2 independent experiments performed in n = 2 technical replicates.

Journal: Nature Microbiology

Article Title: Klebsiella oxytoca inhibits Salmonella infection through multiple microbiota-context-dependent mechanisms

doi: 10.1038/s41564-024-01710-0

Figure Lengend Snippet: ( a-c ) Heatmaps displaying growth displayed as area under curve in selected carbon sources for S . Tm in comparison with K. oxytoca MK01 WT and the sKO mutant after 24 h aerobic incubation using the Biolog microarrays PM1 and PM2a. Sugars are grouped based on their chemical structure with simple sugars and sugar alcohols displayed in ( a ), sugar acids, amines and ketones displayed in ( b ) and amino acids and nucleosides displayed in ( c ). Color scale bar shows AUC over the period of 24 h aerobic incubation. ( a-c ) Results represent mean (average growth) values of n = 3 independent measurements. ( d-g ) Growth curves of S . Tm and MK01 WT and sKO in selected carbon sources including ( d ) dulcitol, ( e ) raffinose, ( f ) sucrose and ( g ) tagatose. Results represent mean ± SEM growth values of n = 3 independent measurements with n = 2 technical replicates. ( h ) S . Tm CFUs and ( i ) K. oxytoca / E. coli CFUs in MM9 medium supplemented with 5 g/l or 10 g/l dulcitol after 24 h of co-cultivation in a 1:1 ratio. Results represent mean ± SEM of n = 2 independent experiments performed in n = 2 technical replicates.

Article Snippet: Extended Data Fig. 9 K. oxytoca and Salmonella have distinct but partially overlapping carbohydrate preferences and likely compete for various substrates. ( a-c ) Heatmaps displaying growth displayed as area under curve in selected carbon sources for S . Tm in comparison with K. oxytoca MK01 WT and the sKO mutant after 24 h aerobic incubation using the Biolog microarrays PM1 and PM2a.

Techniques: Comparison, Mutagenesis, Incubation